Abstract In this experiment, a method for continuous detection of Fe3+and F-based on the fluorescence “OFF-ON” of quinine sulfate was designed. Through the addition of Fe3+, the fluorescence spectrum of quinine sulfate appeared “OFF”. Then, when F-was added, the fluorescence appeared “ON” again. By this method, we can get the quantitative detection of Fe3+and F-continuously. The detection limit of Fe3+is 1.0×10-5mol/L and the linear range is 4×10-5mol/L-8×10-4mol/L. The detection limit of F-is 4.4×10-5mol/L and the linear range is 1.2×10-4mol/L-2.4×10-3mol/L.