化学
血红素
电化学
组合化学
生物传感器
脱氧核酶
粒子(生态学)
DNA
纳米技术
电极
有机化学
酶
血红素
生物化学
物理化学
地质学
海洋学
材料科学
作者
Hao Wang,Cheng Yang,Haoran Tang,Yongxin Li
标识
DOI:10.1021/acs.analchem.0c05055
摘要
Stochastic collision electrochemistry is a hot topic in single molecule/particle research, which provides an opportunity to investigate the details of the single molecule/particle reaction mechanism that is always masked in ensemble-averaged measurements. In this work, we develop an electrochemical amplification strategy to monitor the electrocatalytic behavior of single G-quadruplex/hemin (GQH) for the reaction between hydrogen peroxide and hydroquinone (HQ) through the collision upon a gold nanoelectrode. The intrinsic peroxidase activities of single GQH were investigated by stochastic collision electrochemical measurements, giving further insights into understanding biocatalytic processes. Based on the unique catalytic activity of GQH, we have also designed a hybridization chain reaction strategy to detect miRNA-15 with good selectivity and sensitivity. This work provided a meaningful strategy to investigate the electrochemical amplification and the broad application for nucleic acid sensing at the single molecule/particle level.
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