Comprehensively screening of citric acid ester (CAE) plasticizers in Chinese foodstuffs, and the food-based assessment of human exposure risk of CAEs

An increasing number of studies on the toxicities of citric acid esters (CAEs)-a class of so-called "safe" alternative plasticizers-have highlighted the urgent need to understand their contamination profiles in foodstuffs and the corresponding potential risks to human health. This study determined the concentrations of 8 target CAEs in 105 foodstuff samples, grouped into 6 food categories, collected from Nanjing City, China, in 2020. All eight CAEs were detected in at least one of the analyzed samples and had detection frequencies (DFs) of 5-47%. The DFs and distribution profiles of the target CAEs varied among different food categories; for example, cereals had the highest DF (92%), while meat/fish contained the highest mean total concentration of CAEs (8.35 ng/g wet weight (ww)). Among the target CAEs, acetyl tributyl citrate (ATBC) had the highest DF (47%), and tributyl citrate (TBC) exhibited the highest mean concentration (1.24 ng/g ww). Based on the food ingestion route, the estimated total daily intake (EDI) values of the target CAEs for adults under average- and high-exposure scenarios were 38.3 ng/kg of body weight (bw) and 111 ng/kg bw, respectively, which were attributed to the high percentage contributions of TBC (50.6%) and ATBC (23.7%). In addition, a suspect and characteristic fragment-dependent screening strategy was applied to the foodstuff data, and a novel CAE, monoethyl citrate (MEC, CAS: 4552-00-5), with a DF of 34% was tentatively identified. Overall, this study provides novel and comprehensive information regarding the pollution status of CAEs in foodstuffs.