胶束
乙二醇
乳酸
生物相容性
共聚物
核化学
药物输送
化学
PEG比率
临界胶束浓度
MTT法
质子核磁共振
高分子化学
体外
水溶液
生物化学
有机化学
聚合物
生物
经济
细菌
遗传学
财务
作者
Qinglai Yang,Changyu He,Zhen Zhang,Lianjiang Tan,Bingya Liu,Zhenggang Zhu,Zhifeng Shao,Bing Gong,Yu‐Mei Shen
出处
期刊:Polymer
[Elsevier]
日期:2016-05-01
卷期号:90: 351-362
被引量:29
标识
DOI:10.1016/j.polymer.2016.03.030
摘要
Redox-responsive micelles self-assembled from triblock copolymers of poly(l-lactic acid)-poly(ethylene glycol)-poly(l-lactic acid) (PLA-PEG-PLA) with double-disulfide linkage in the backbone were synthesized and characterized by proton nuclear magnetic resonance (1H NMR) and size exclusion chromatography (SEC), in which both PEG (Mn = 1000, 2000 and 4000 g mol−1) and PLA (Mn = 1600 g mol−1) have different molecular weights respectively. The triblock copolymers PLA3000-PEG2000-PLA3000 and PLA3000-PEG4000-PLA3000 can self-assemble into flower-like micelles in aqueous media with average diameters 110 nm and 43 nm and lower critical micelle concentrations (CMC) 0.017 and 0.014 mg mL−1 respectively compared with that of diblock copolymers. Moreover, in vitro drug release analyses indicated that reductive environment can result in triggered drug release profiles. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl- 2-H-tetrazolium bromide (MTT) assay in vitro showed no significant cytotoxicity as NIH 3T3 cells incubated in the micelles even when the concentrations up to 1000 μg/mL. Additionally fluorescence microscopy measurements and MTT assay demonstrated that the micelles exhibited a faster drug release and higher cellular proliferation inhibition due to the effect of intracellular reduction responsiveness compared with that of diblock copolymers. The above results suggest that the reduction-responsive, biodegradable and biocompatibility micelles could provide a platform to construct potential drug delivery systems for cancer therapy.
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