High-speed preparative counter-current chromatography with a coil planet centrifuge

A compact table-top model of a flow-through coil planet centrifuge produces and efficient chromatagraphic separation of solutes on a preparative scale within 2–5 h. The use of a multi-layer coiled column promotes retention of the stationary phase with a high flow-rate of the mobile phase and permits almost universal application of conventional two-phase solvent systems. The capability of the counter-current chromatography scheme has been demonstrated on separations of various biological samples, such as dinitrophenyl amino acids, dipeptides, gramicidins, auxins, purines and pyrimidines.