生物
胶质纤维酸性蛋白
细胞生物学
表皮生长因子
内斯汀
星形胶质细胞
表皮生长因子受体
受体
分子生物学
神经干细胞
免疫学
神经科学
生物化学
干细胞
免疫组织化学
中枢神经系统
作者
Ee Ling Ng,Jun Jie Ng,Fengyi Liang,Bor Luen Tang
摘要
The expression profile and functions of the brain-enriched Rab22B/Rab31 small GTPase had remained uncharacterized. Using specific antibodies against Rab22B, we found the protein to be exceptionally enriched in nestin and RC2-positive radial glia of the embryonic mouse brain. In the adult brain, Rab22B is rather specifically expressed in glial fibrillary acidic protein (GFAP)-positive mature astrocytes, but is not clearly detectable in either 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase)-positive mature oligodendrocytes or βIII-tubulin (TuJ)-positive neurons. In probing for specific functions of Rab22B, we found that Rab22B silencing in A431 cells resulted in abnormal trafficking of the epidermal growth factor receptor (EGFR), Texas-red-labeled EGF, and the cation-independent mannose 6-phosphate receptor (M6PR). Affinity pull-down assays and co-immunoprecipitation analysis indicated that Rab22B could associate with EGFR in a GTP-dependent manner. Rab22B is thus a Rab protein specifically expressed in the astroglia lineage and may have a role in regulating EGFR trafficking in some cell types. Given that EGFR signaling modulates astrocyte development and oncogenesis of multiple cell types, Rab22B may thus have specific developmental or pathophysiological roles in cell types which it is enriched in. J. Cell. Physiol. 221: 716–728, 2009. © 2009 Wiley-Liss, Inc.
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