体外
病毒复制
化学
病毒学
病毒
传染性
突变体
阿昔洛韦
生物
生物化学
疱疹病毒科
基因
病毒性疾病
作者
Charles C. Buck,Philip C. Loh
标识
DOI:10.1016/0166-3542(85)90041-5
摘要
The antiviral effect of acyclovir (ACV; 9-(2-hydroxyethoxymethyl)guanine) on the replication of channel catfish virus (CCV), a poikilothermic herpesvirus, in brown bullhead cells (BB) was studied in vitro. Acyclovir at concentrations of 2 μM and 10 μM produced a 95 to 99% reduction in plaque numbers, respectively. At 10 μM ACV did not affect the growth of uninfected BB cells which could even be subcultured for four passages in the presence of the inhibitor. To be effective ACV had to be added early in the infectious cycle and was progressively less effective when added at later times after infection. Similar to homeothermic herpesviruses, the inhibition could be reversed with the addition of excess thymidine. The effects of three other antiviral agents, adenine-β-d-arabinofuranoside (ara-A), phosphonoacetic acid (PAA), and phosphonoformic acid (PFA) on CCV replication were evaluated either individually or in combination with ACV. Although the other agents were found to be less effective in inhibiting CCV replication than ACV, none of the agents interacted synergistically with ACV. The results indicated that interactions with ACV with ara-A, PFA and PAA were primarily additive. Mutants of CCV resistant to ACV were obtained and were found to be somewhat more resistant to PFA than was the stock CCV.
科研通智能强力驱动
Strongly Powered by AbleSci AI