Relationship between the concentration and specific gravity, and refractive index were measured in the systems of water-glycol, glycol-diethylene glycol, and diethylene glycol-triethylene glycol. The concentration-specific gravity relationship gave a curve with a very slight bend while the relationship between concentration and refractive index was linear. It was found possible, therefore, to calculate the concentration, x, of each component by the measurement of a refractive index, n, of a mixture of two substances. Following empirical formulae for each system were proposed.Water-Glycol: glycol%, x=1006.0n-1341.0.Glycol-Diethylene glycol: diethylene glycol%, x=6493.5n-9301.3.Diethylene glycol-Triethylene glycol: triethylene glycol%, x=10526.3n-15240.0