Pharmacognostic, Phytochemical and Hepatoprotective Activity of Berries of Vitex agnus- castus

The aim of this study was to assess the hepatoprotective properties of several Vitex agnus-castus berry extracts in vitro utilizing a normal Chang liver cell line and the MTT test.Pharmacognostical investigations identified a number of distinctive traits found in the plant.The ethyl acetate extract yielded a larger percentage than the other extracts when the berries of Vitex agnus-castus were extracted using solvents with increasing polarity.The antioxidant activity was determined using the reducing power ability assay and the H2O2 scavenging method.The ethyl acetate extract showed the highest antioxidant activity compared to the other two extracts; in all processes, its IC50 value was almost equivalent to that of the reference drug ascorbic acid.The highest level of protection against paracetamol-induced hepatotoxicity was provided by the ethyl acetate extract, according to in vitro hepatoprotective experiments conducted on Chang liver cell line.Our research confirmed the hypothesis that Vitex agnus-castus may be a beneficial hepatoprotective herb.It is recommended to conduct more research on identifying the phytoconstituents causing the action.