Novel Polypeptide Automated Synthesis Devices: A Review

Peptides are a unique class of proteins and one-dimensional biological nanomaterials that play an exceptionally important role in life science and industrial applications. At present, peptide synthesis equipment encounters issues like low synthetic efficiency, challenges in scaling up, and limited automation. This article provides an overview of the key technologies in peptide synthesis equipment, covering aspects such as peptide information screening, peptide synthesis, and detection. It analyzes and summarizes the principles, methods, and critical challenges of traditional solid-phase peptide synthesis technology, microwave-assisted solid-phase peptide synthesis technology, and continuous-flow solid-phase peptide synthesis technology. It also discusses microfluidic solid-phase peptide synthesis technology and future research trends, offering insights into the search for an efficient, high-yield, high-purity, scalable, and intelligent peptide synthesis method, which holds significant research significance.