Possibilities of application of Raman spectroscopy in phenotyping of patients with sepsis (literature review)

Introduction. Sepsis remains one of the leading causes of mortality in intensive care units. The heterogeneity of the population of these patients determines the need to search for new technologies for stratification of its severity. One of these methods is Raman spectroscopy, which allows to perform molecular analysis of biological fluids without lengthy sample preparation. The objective was to analyze the possibilities of using Raman spectroscopy for phenotyping of patients with sepsis. Materials and methods. A review of current studies in which Raman spectroscopy has been used to identify biomarkers of inflammation, bacterial pathogens, and to assess the immune response in patients with suspected sepsis. Results. It is noted that the Raman spectroscopy technology demonstrates high sensitivity and specificity in the diagnosis of infectious processes, including the determination of antibiotic sensitivity and real-time monitoring of treatment. The main limitations of the method remain the high cost of equipment and the need for qualified personnel. Conclusion. The development of portable systems and algorithms for automatic spectral processing contributes to the expansion of the clinical application of Raman spectroscopy.