脑出血
神经外科
生物标志物
医学
白质
血管外科
神经化学
神经学
神经组阅片室
重症监护医学
心脏外科
外科
内科学
磁共振成像
放射科
蛛网膜下腔出血
精神科
生物化学
化学
作者
Yi Zhang,Hanhai Zeng,Feiyang Lou,Xiaoxiao Tan,Xiaolin Zhang,Gao Chen
标识
DOI:10.1007/s12975-023-01145-5
摘要
Abstract Intracerebral hemorrhage (ICH) is a severe cerebrovascular disease, which impairs patients’ white matter even after timely clinical interventions. Indicated by studies in the past decade, ICH-induced white matter injury (WMI) is closely related to neurological deficits; however, its underlying mechanism and pertinent treatment are yet insufficient. We gathered two datasets (GSE24265 and GSE125512), and by taking an intersection among interesting genes identified by weighted gene co-expression networks analysis, we determined target genes after differentially expressing genes in two datasets. Additional single-cell RNA-seq analysis (GSE167593) helped locate the gene in cell types. Furthermore, we established ICH mice models induced by autologous blood or collagenase. Basic medical experiments and diffusion tensor imaging were applied to verify the function of target genes in WMI after ICH. Through intersection and enrichment analysis, gene SLC45A3 was identified as the target one, which plays a key role in the regulation of oligodendrocyte differentiation involving in fatty acid metabolic process, etc. after ICH, and single-cell RNA-seq analysis also shows that it mainly locates in oligodendrocytes. Further experiments verified overexpression of SLC45A3 ameliorated brain injury after ICH. Therefore, SLC45A3 might serve as a candidate therapeutic biomarker for ICH-induced WMI, and overexpression of it may be a potential approach for injury attenuation. Graphical Abstract
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