High-purity alkaline lignin extraction from Saccharum ravannae and optimization of lignin recovery through response surface methodology

Saccharum ravannae, known as "Ekra" in the Northeast region of India, is an elephant grass species that abundantly grows in the natural habitat of Assam. This study aims to utilize this wild grass species and extract alkaline lignin of high purity through KOH-mediated alkaline hydrothermal pretreatment using the Oil bath process. Lignin recovery was optimized using RSM (response surface methodology) combined with a central composite model. Three process parameters, namely KOH concentration (1-3 %), reaction time (50-200 min), and solid loading (5-15 %), varied to optimize the combined effect of these parameters. RSM predicted a maximum lignin recovery of 15.38 g/100 g of raw biomass at optimum conditions (2.4 % KOH, 6.41 % solid loading, 176.57 min). Three experimental runs were performed at optimum conditions, and 15.81 ± 0.32 g/100 g lignin recovery was obtained, thus verifying the predicted result. Maximum 93.7 % purity of extracted lignin was achieved in a different operating condition (3 % KOH, 10 % solid loading, 125 min). The commercial and extracted alkaline lignin with maximum purity was characterized by Nuclear Magnetic Resonance (NMR), Fourier Transform Infrared Spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). The extracted lignin shows higher phenolic content and more functional groups than commercial lignin and can be used for future applications.