RNA molecules undergo extensive modifications by enzymes known as RNA modification enzymes. Over 170 different types of these post-transcriptional modifications have been identified,1 and it is expected that this number will continue to grow. The field of RNA modification research has experienced a resurgence, driven by mounting evidence highlighting its crucial role in gene expression regulation. Among these modifications is N4-acetylcytidine (ac4C), a highly conserved modification found across all domains of life.