溴脱氧尿苷
神经发生
纽恩
齿状回
海马体
细胞生长
胶质发生
纹状体
生物
大脑皮层
细胞分化
增殖标记
细胞生物学
神经科学
皮质(解剖学)
免疫组织化学
免疫学
神经干细胞
干细胞
遗传学
多巴胺
基因
作者
Lin Qiu,Changlian Zhu,Xiaoyang Wang,Fanxing Xu
标识
DOI:10.1007/s12264-007-0007-0
摘要
To investigate the cell proliferation and differentiation in the developing brain of mouse.C57/BL6 mice were divided into 3 groups at random. Bromodeoxyuridine (BrdU) was injected into the brains in different development periods once a day for 7 d. The brains were retrieved 4 weeks after the last BrdU injection. Immunohistochemical and immunofluorescent studies were carried out for detecting cell proliferation (BrdU) and cell differentiation (NeuN, APC, Iba1, and S100beta), respectively.The number of BrdU labeled cells decreased significantly with the development of the brain. Cell proliferation was prominent in the cortex and striatum. A small portion of BrdU and NeuN double labeled cells could be detected in the cortex at the early stage of development, and in the striatum and CA of the hippocampus in all groups. The majority of BrdU labeled cells were neuroglia, and the number of neuroglia cells decreased dramatically with brain maturation. Neurogenesis is the major cytogenesis in the dentate gyrus.These results demonstrated that cell proliferation, differentiation and survival were age and brain region related.
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