Bacillus subtilis and Bacillus licheniformis in diets for Nile tilapia ( Oreochromis niloticus ): Effects on growth performance, gut microbiota modulation and innate immunology

Aquaculture ResearchVolume 52, Issue 4 p. 1630-1642 ORIGINAL ARTICLE Bacillus subtilis and Bacillus licheniformis in diets for Nile tilapia (Oreochromis niloticus): Effects on growth performance, gut microbiota modulation and innate immunology Leonardo Tachibana, Corresponding Author tachibana@pesca.sp.gov.br orcid.org/0000-0002-5847-8723 Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, Brazil Correspondence Leonardo Tachibana, Aquaculture Research Center. Scientific research of Fisheries Institute/APTA/SAA, São Paulo. Av. Francisco Matarazzo, 455 - 05001-900 São Paulo, Brazil. Email: ltachibana@sp.gov.brSearch for more papers by this authorGuilherme Silveira Telli, Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this authorDanielle de Carla Dias, orcid.org/0000-0002-8375-5948 Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this authorGiovani Sampaio Gonçalves, Scientific Research of Advanced Technological Research Center for Continental Fishery Agribusiness of Fisheries Institute/APTA/SAA, São José do Rio Preto, BrazilSearch for more papers by this authorMateus Cardoso Guimarães, orcid.org/0000-0002-3169-2367 Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this authorCarlos Massatoshi Ishikawa, Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this authorRaissa Bertoncello Cavalcante, Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this authorMariene Miyoko Natori, orcid.org/0000-0003-0471-0096 Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this authorMiguel Frederico Fernandez Alarcon, orcid.org/0000-0001-7206-2209 Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this authorSilvana Tapia-Paniagua, Department of Microbiology, Malaga University, Málaga, SpainSearch for more papers by this authorMiguel Ángel Moriñigo, Department of Microbiology, Malaga University, Málaga, SpainSearch for more papers by this authorFrancisco Javier Moyano, orcid.org/0000-0002-0754-1809 Department of Biology and Geology, Almeria University, Almeria, SpainSearch for more papers by this authorEdnara Ronise Lima de Araújo, Laboratory of Estuarine and Marine Fish Culture, Federal University of Rio Grande, Institute of Oceanography, Rio Grande, RS, BrazilSearch for more papers by this authorMaria José T. Ranzani-Paiva, orcid.org/0000-0001-8279-047X Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this author Leonardo Tachibana, Corresponding Author tachibana@pesca.sp.gov.br orcid.org/0000-0002-5847-8723 Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, Brazil Correspondence Leonardo Tachibana, Aquaculture Research Center. Scientific research of Fisheries Institute/APTA/SAA, São Paulo. Av. Francisco Matarazzo, 455 - 05001-900 São Paulo, Brazil. Email: ltachibana@sp.gov.brSearch for more papers by this authorGuilherme Silveira Telli, Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this authorDanielle de Carla Dias, orcid.org/0000-0002-8375-5948 Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this authorGiovani Sampaio Gonçalves, Scientific Research of Advanced Technological Research Center for Continental Fishery Agribusiness of Fisheries Institute/APTA/SAA, São José do Rio Preto, BrazilSearch for more papers by this authorMateus Cardoso Guimarães, orcid.org/0000-0002-3169-2367 Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this authorCarlos Massatoshi Ishikawa, Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this authorRaissa Bertoncello Cavalcante, Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this authorMariene Miyoko Natori, orcid.org/0000-0003-0471-0096 Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this authorMiguel Frederico Fernandez Alarcon, orcid.org/0000-0001-7206-2209 Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this authorSilvana Tapia-Paniagua, Department of Microbiology, Malaga University, Málaga, SpainSearch for more papers by this authorMiguel Ángel Moriñigo, Department of Microbiology, Malaga University, Málaga, SpainSearch for more papers by this authorFrancisco Javier Moyano, orcid.org/0000-0002-0754-1809 Department of Biology and Geology, Almeria University, Almeria, SpainSearch for more papers by this authorEdnara Ronise Lima de Araújo, Laboratory of Estuarine and Marine Fish Culture, Federal University of Rio Grande, Institute of Oceanography, Rio Grande, RS, BrazilSearch for more papers by this authorMaria José T. Ranzani-Paiva, orcid.org/0000-0001-8279-047X Aquaculture Research Center, Scientific Research of Fisheries Institute/APTA/SAA, São Paulo, BrazilSearch for more papers by this author First published: 25 November 2020 https://doi.org/10.1111/are.15016Citations: 1 Funding information: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) [grant number 2013/09731-8]; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) [grant number: 470270/2013-6]. [Corrections added on 15 December 2020, after first online publication: Co-author Ednara Ronise Lima de Araújo has been added.] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinked InRedditWechat Abstract This study aimed to verify the possible positive effects of feeding the commercial probiotic Bioplus 2BC® [Bacillus subtilis (1.6 × 1010 CFU/g) and Bacillus licheniformis (1.6 × 1010 CFU/g)] to Nile tilapia on its growth performance, nutrient digestibility, gut microbiology, haematology (hematimetric index, erythrocyte, leucocyte and thrombocyte counts) and innate immunology (lysozyme, burst respiratory and phagocyte activities). For this purpose, one in vivo assay was performed following a completely randomized design, with four treatments (0.00%; 0.02%; 0.04%; and 0.08% of probiotic inclusion on ratio) and four replicates. The fish fed with diets containing 0.04% and 0.08% of probiotics presented higher weight gain than the control group, as well as higher thrombocyte counts in the bloodstream. The positive effect of the probiotics on the gut microbiota was illustrated by the higher richness and habitability indexes, soaring genetic variability. On the contrary of control, the groups fed with probiotic had Firmicutes, Fusobacteria and Bacteroidetes phyla, and a lower percentage of Proteobacteria. The inclusion of 0.04% and 0.08% of B. subtilis and B. licherniformis in the fed provides better growth performance and modifies the intestinal microbiota, reducing the class of potential pathogen agent species. The establishment of a beneficial microorganism population may improve host health. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are available upon the request. Citing Literature Supporting Information Filename Description are15016-sup-0001-TablesS1-S4.pdfPDF document, 57 KB Tables S1-S4 Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume52, Issue4April 2021Pages 1630-1642 RelatedInformation