Abstract We present advancements in an enhanced temperature measurement technique using NV centers in diamond with an orthogonal orientation of bias magnetic file. This approach reduces magnetic noise interference and improves sensitivity, particularly in biocompatible conditions. We systematically evaluate the sensitivity of the diamond-based temperature sensor and demonstrate up one order improved sensitivity at low laser power for the “super transverse” bias magnetic field (S-TBMF) regime. This approach simplifies the experimental setup, provides remarkable magnetic noise decoupling, and can be extended to temperature sensing using nanodiamonds (NDs).