氨单加氧酶
羟胺
生物
亚硝酸盐
氨
序批式反应器
硝化作用
欧洲亚硝基单胞菌
反硝化
微生物学
碱性磷酸酶
粪碱杆菌
基因簇
生物化学
操纵子
废水
硝酸盐
拉伤
基因
细菌
化学
氮气
大肠杆菌
遗传学
生态学
有机化学
古细菌
废物管理
工程类
解剖
假单胞菌
作者
Miao Wu,Tingting Hou,Ying Liu,Lili Miao,Guomin Ai,Li Ma,Hai-Zhen Zhu,Yaxin Zhu,Xiyan Gao,Craig W. Herbold,Michael Wagner,Defeng Li,Zhi-Pei Liu,Shuang‐Jiang Liu
标识
DOI:10.1111/1462-2920.15751
摘要
Summary Heterotrophic nitrifiers are able to oxidize and remove ammonia from nitrogen‐rich wastewaters but the genetic elements of heterotrophic ammonia oxidation are poorly understood. Here, we isolated and identified a novel heterotrophic nitrifier, Alcaligenes ammonioxydans sp. nov. strain HO‐1, oxidizing ammonia to hydroxylamine and ending in the production of N 2 gas. Genome analysis revealed that strain HO‐1 encoded a complete denitrification pathway but lacks any genes coding for homologous to known ammonia monooxygenases or hydroxylamine oxidoreductases. Our results demonstrated strain HO‐1 denitrified nitrite (not nitrate) to N 2 and N 2 O at anaerobic and aerobic conditions respectively. Further experiments demonstrated that inhibition of aerobic denitrification did not stop ammonia oxidation and N 2 production. A gene cluster ( dnfT1RT2ABCD ) was cloned from strain HO‐1 and enabled E . coli accumulated hydroxylamine. Sub‐cloning showed that genetic cluster dnfAB or dnfABC already enabled E . coli cells to produce hydroxylamine and further to 15 N 2 from ( 15 NH 4 ) 2 SO 4 . Transcriptome analysis revealed these three genes dnfA , dnfB and dnfC were significantly upregulated in response to ammonia stimulation. Taken together, we concluded that strain HO‐1 has a novel dnf genetic cluster for ammonia oxidation and this dnf genetic cluster encoded a previously unknown pathway of di rect amm onia ox idation (Dirammox) to N 2 .
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