细胞凋亡
DNA断裂
细胞毒性T细胞
细胞毒性
异烟肼
化学
细胞周期
MTT法
免疫系统
细胞生长
癌症研究
MCF-7型
碎片(计算)
细胞周期检查点
程序性细胞死亡
药理学
癌细胞
分子生物学
免疫学
癌症
生物
医学
肺结核
生物化学
体外
病理
内科学
人体乳房
生态学
作者
Muttiah Barathan,Ahmad Khusairy Zulpa,Kumutha Malar Vellasamy,Vanitha Mariappan,Naveen Kumar Hawala Shivashekaregowda,Zaridatul Aini Ibrahim,Jamuna Vadivelu
出处
期刊:in Vivo
[Anticancer Research USA Inc.]
日期:2021-01-01
卷期号:35 (5): 2675-2685
被引量:3
标识
DOI:10.21873/invivo.12551
摘要
Background/Aim: Isoniazid is an antibiotic used for the treatment of tuberculosis. Previously, we found that the isoniazid derivative (E)-N'-(2,3,4-trihydroxybenzylidene) isonicotinohydrazide (ITHB4) could be developed as novel antimycobacterial agent by lead optimization. We further explored the ability of this compound compared to zerumbone in inhibiting the growth of MCF-7 breast cancer cells. Materials and Methods: Cytotoxicity was measured by the MTT assay and further confirmed via apoptosis, ROS, cell cycle, DNA fragmentation and cytokine assays. Results: ITHB4 demonstrated a lower IC50 compared to zerumbone in inhibiting the proliferation of MCF-7 cells. ITHB4 showed no toxicity against normal breast and human immune cells. Apoptosis assay revealed that ITHB4, at a concentration equal to the IC50, induces apoptosis of MCF-7 cells and cell cycle arrest at the sub-G1 and G2/M phases. ITHB4 triggered accumulation of intracellular ROS and nuclear DNA fragmentation. Secretion of pro-inflammatory cytokines induced inflammation and potentially immunogenic cell death. Conclusion: ITHB4 has almost similar chemotherapeutic properties as zerumbone in inhibiting MCF-7 growth, and hence provide the basis for further experiments in animal models.
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