Updating the MISEV minimal requirements for extracellular vesicle studies: building bridges to reproducibility

An editorial describing “minimal experimental requirements for definition of extracellular vesicles (EVs)”, or\nmore simply “minimal information for studies of EVs (MISEV)” was published in the Journal of Extracellular\nVesicles in late 2014 [1]. Similar to guidelines in other scientific fields [2–4], “MISEV2014”, as we will call it\nhere, provided recommendations on experimental methods and minimal information in reporting. Specifically, three key areas were addressed: EV isolation/purification, EV characterization and EV functional studies (see Text Box 1).