水平基因转移
质粒
碘化丙啶
抗生素耐药性
流式细胞术
基因
抗生素
细菌
微生物学
大肠杆菌
生物物理学
化学
生物
分子生物学
遗传学
生物化学
细胞凋亡
程序性细胞死亡
系统发育树
作者
Xiaolong Wang,Fengxia Yang,Jing Zhao,Yan Xu,Daqing Mao,Xiao Zhu,Yi Luo,Pedro J. J. Alvarez
出处
期刊:NanoImpact
[Elsevier]
日期:2018-04-01
卷期号:10: 61-67
被引量:104
标识
DOI:10.1016/j.impact.2017.11.006
摘要
Bacterial exposure to ZnO nanoparticles (nZnO) at sublethal concentrations (1 to 10 mg/l) for 24 h significantly increased the conjugative frequency of antibiotic resistance plasmid RP4. A 24.3-fold increase was observed in E. coli pure cultures, and an 8.3-fold increase occurred in a mixed culture of indigenous aquatic microbiota. In addition, nZnO increased by three fold the transformation efficiency of E. coli via the uptake of naked plasmid pGEX4T-1, which was confirmed by confocal fluorescence microscopy. Enhanced horizontal transfer of resistance plasmids was a nanoparticle-specific effect, since 5.3 mg/l Zn2 + (representing the maximum release of Zn2 + from 10 mg/l nZnO) did not enhance RP4 transfer or pGEX4T-1 uptake frequency. This phenomenon was attributed to increased bacterial cell permeability after exposure to nZnO (but not to Zn2 +), which was confirmed by flow cytometry with propidium iodide staining. Overall, this study forewarns that accidental or incidental release of nZnO may facilitate environmental dissemination and propagation of antibiotic resistance genes through horizontal gene transfer.
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