自噬
线粒体
细胞毒性
活性氧
流式细胞术
癌细胞
生物物理学
材料科学
共焦显微镜
细胞凋亡
细胞生物学
细胞
细胞内
荧光显微镜
化学
癌症
生物
荧光
生物化学
体外
分子生物学
遗传学
物理
量子力学
作者
Ya-Na Wu,Li-Xing Yang,Xuan-Yu Shi,I-Chen Li,Joanna M. Biazik,Kyle R. Ratinac,Dong Hwang Chen,Pall Thordarson,Dar-Bin Shieh,Filip Braet
出处
期刊:Biomaterials
[Elsevier]
日期:2011-07-01
卷期号:32 (20): 4565-4573
被引量:132
标识
DOI:10.1016/j.biomaterials.2011.03.006
摘要
Nanoparticles with an iron core and gold shell (denoted "Fe@AuÓ") have been reported to limit cancer-cell proliferation and therefore have been proposed as a potential anti-cancer agent. However, the underlying mechanisms are still unknown. In this study, we used flow cytometry, confocal fluorescence microscopy, and transmission electron microscopy to analyse the morphological and functional alterations of mitochondria in cancerous cells and healthy cells when treated with Fe@Au. It was found that Fe@Au caused an irreversible membrane-potential loss in the mitochondria of cancer cells, but only a transitory decrease in membrane potential in healthy control cells. Production of reactive oxygen species (ROS) was observed; however, additions of common ROS scavengers were unable to protect cancerous cells from the Fe@Au-induced cytotoxicity. Furthermore, iron elements, before oxidation, triggered mitochondria-mediated autophagy was shown to be the key factor responsible for the differential cytotoxicity observed between cancerous and healthy cells.
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