肠系膜淋巴结
拟杆菌
结肠炎
免疫学
炎症
化学
干扰素γ
细胞因子
分子生物学
生物
免疫系统
细菌
遗传学
作者
Frank Hoentjen,Susan L. Tonkonogy,Bi-Feng Qian,Bo Liu,Levinus A. Dieleman,R. Balfour Sartor
出处
期刊:Inflammatory Bowel Diseases
[Oxford University Press]
日期:2007-03-01
卷期号:13 (3): 317-324
被引量:23
摘要
HLA-B27/β2 microglobulin transgenic (TG) rats develop spontaneous colitis when raised under specific pathogen-free (SPF) conditions or after monoassociation with Bacteroides vulgatus (B. vulgatus), whereas germ-free TG rats fail to develop intestinal inflammation. SPF HLA-B27 TG rnu/rnu rats, which are congenitally athymic, remain disease free. These results indicate that commensal intestinal bacteria and T cells are both pivotal for the development of colitis in TG rats. However, it is not known if T cells are also required in the induction of colitis by a single bacterial strain. The aim of this study was therefore to investigate the role of T cells in the development of colitis in B. vulgatus–monoassociated HLA-B27 TG rats. HLA-B27 TG rnu/rnu and rnu/+ rats were monoassociated with B. vulgatus for 8–12 weeks. CD4+ T cells from mesenteric lymph nodes (MLNs) of B. vulgatus–monoassociated rnu/+ TG donor rats were transferred into B. vulgatus–monoassociated rnu/rnu TG recipients. B. vulgatus–monoassociated rnu/+ rats showed higher histologic inflammatory scores and elevated colonic interferon-γ mRNA, cecal myeloperoxidase, and cecal IL-1β levels compared to those in rnu/rnu TG rats that did not contain T cells. After transfer of CD4+ cells from colitic B. vulgatus–monoassociated rnu/+ TG donor rats, B. vulgatus–monoassociated rnu/rnu TG recipients developed colitis that was accompanied by B. vulgatus-induced IFN-γ production by MLN cells in vitro and inflammatory parameters similar to rnu/+ TG rats. These results implicate CD4+ T cells in the development of colitis in HLA-B27 TG rats monoassociated with the nonpathogenic bacterial strain B. vulgatus.
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