Directed evolution of enzymes: Library screening strategies

Abstract Directed evolution has become the preferred engineering approach to generate tailor‐made enzymes. The method follows the design guidelines of nature: Darwinian selection of genetic variants. This review discusses the different stages of directed evolution experiments with the focus on developments in screening and selection procedures. © 2009 IUBMB IUBMB Life 61(3): 222–228, 2009