Effects of containing drugs blood serum of donkey - hide glue reinforcing bone oral solution on mRNA expressing of OPG and OPGL in osteoblast in rats

Objective To investigate the effects of containing drugs blood serum of donkey - hide glue reinforcing bone oral solution (DGRBOS) on mRNA expression of OPG and OPGL in osteoblast in rats and explore the molecular mechanism of treating osteoporosis with DGRBOS. Methods 30 Wistar rats were randomly divided into DGRBOS group, estrogen group and normal control group. After intragastric administration for 7 days, containing drugs serum was prepared, Skull osteoblast was isolated from newborn SD rats and was made into single cell suspension. The cultured osteoblasts were divided into 5 groups. The experimental groups were given equal volumes of drugs serum and the control group was given cultured fluid. The osteoblast proliferation was measured by antigenic MTT colorimetric analysis and 3 H - TdR penetration method. The intra - cellular BGP contents were evaluated by radioimmunity. The mRNA expression of OPG and PANKL in osteoblasts was analyzed by Rt -PCR. Results DGRBOS could enhance osteoblast proliferation in a dose - dependent manner, compared to the control group; a significant difference was present (P0. 05). As the concentration of contained drug serum of DGRBOS for 1000ml/L appeared as the peak of mRNA expression OPG and OPGL in osteoblasts, compared with control group, significant differences were absent (P0. 05). Conclusion The partial mechanism of preventing and treating osteoporosis with DGRBOS is that it might promote osseous anabolism and preosteoblast caryocinesia at cellular lever and regulate OPG/ RANKL ratio at molecular lever.