Research of the apoptosis of TRAIL on cervical cancer HeLa cells

Objective To observe the effects of different concentration of TRAIL on cervical cancer HeLa cells.Methods With different concentration of TRAIL role in HeLa cells,the survival fraction of HeLa cells was measured by MTT assay in Chongqing Medical University in 2006.Structures of apoptosis cells were observed by AO/EB fluorescence microscopy.Cell cycle of HeLa cells and apoptosis rates were determined by FACS.Results After treating HeLa cells for 24 h by TRAIL,a good dose-effect relationship was demonstrated by the role of TRAIL in the rate of cell growth inhibition and apoptosis;early and late apoptosis cell can be seen by AO/EB stainning and the hypodiploid peaks were found by FACS checking.The cell cycles were changed.Conclusion The proliferation of HeLa cells can be inhibited and the apoptosis can be induced by TRAIL.