Research on cytotoxicity of cisplatin combined with carboplatin in osteosarcoma cell line by using the MTT assay

Objective:To provide experimental proofs for cisplatin combined with carboplatin in therapy of osteosarcoma through comparing the cytotoxity of half dose cisplatin combined with half dose carboplatin,cisplatin and arboplatin in osteosarcoma cell line(OS-732).Methods:OS-732 cells were treated by IC 50 of cisplatin,IC 50 of carboplatin,or 1/2 IC 50 cisplatin combined with 1/2 IC 50 carboplatin,and cultured 48h or 72h.Then their viability was determined by the colorimetric MTT assay.Results:When cells were cultured for 48hrs,cytotoxicities of group IC 50 cisplatin,IC 50 carboplatin and 1/2 IC 50 cisplatin+1/2 IC 50 carboplatin for OS-732 cells had no significant difference( P 0 05);when cells were cultured for 72 hrs,cytotoxicities of group IC 50 carboplatin and group 1/2 IC 50 cisplatin+1/2 IC 50 carboplatin for OS-732 cells were higher than that of group IC 50 cisplatin( P 0 05)Conclusion:Cisplatin combined with carboplatin as a “single agent” appears to be as active as cisplatin or carboplatin alone in