Objective To compare the difference in the effect of eliminating lipid interference in biochemical examination between dilution with deionized water and high-speed centrifugation.Methods The appropriate dilution with deionized water by triacylglycerol interference test was selected.Dilution with deionized water and high-speed centrifugation(13000 r/m,10 min) were compared in the effect of eliminating lipid interference in biochemical examination and analyzed using paired T-test.Results ALT,AST,CK,CKMB,CREA,UREA,GLU,AMY,and LPS results by dilution with deionized water were consistent with those by high-speed centrifugation.The difference was not statistically significant.Conclusions Dilution with deionized water can eliminate the impact of lipids and provide clinicians with accurate biochemical test results.