The preparation of Fe-based peroxidase mimetic nanozymes and for the electrochemical detection of histamine

Histamine is one of the main triggers of aquatic allergies. In this work, a sensitive, selective and inexpensive electrochemical biosensor for the detection of histamine was developed. The DNA aptamer highly selective for histamine was covalently conjugated on gold nanoflower-modified ITO electrode as the working electrode (Apt/AuNFs/ITO), while [email protected] nanohybrids with peroxidase mimetic activity was used as electrochemical probe. The amount of probe linked to the electrode surface dependent on histamine concentration, and could be indicated by differential pulse voltammetry method in TMB-H2O2 system, thus paving a way for histamine sensing. This electrochemical biosensor exhibited a wide detection range from 1 to 5000 nmol L-1, and a low detection limit (LOD) of 0.79 nmol L-1. Furthermore, the sensor was successfully applied to detect histamine in real crab samples, indicating a good application prospect in actual sample determination.