免疫系统
佐剂
表位
生物
肽
病毒学
免疫
T细胞
抗体
先天免疫系统
免疫
免疫学
接种疫苗
细胞
免疫耐受
获得性免疫系统
抗原
细胞毒性T细胞
CpG站点
分子生物学
细胞免疫
B细胞
RGD基序
体液免疫
肽疫苗
肽序列
作者
Martine Bruley Rosset,Clara Ballerini,Sylvie Grégoire,Pat Metharom,Claude Carnaud,Pièrre Aucouturier
出处
期刊:Journal of Immunology
[American Association of Immunologists]
日期:2004-05-01
卷期号:172 (9): 5168-5174
被引量:62
标识
DOI:10.4049/jimmunol.172.9.5168
摘要
Abstract The absence of a detectable immune response during transmissible spongiform encephalopathies is likely due to the fact that the essential component of infectious agents, the prion protein (PrP), is a self Ag expressed on the surface of many cells of the host. To overcome self-tolerance to PrP, we used 30-mer PrP peptides previously shown to be immunogenic in Prnp−/− mice, together with CFA or CpG-oligodeoxynucleotides (CpG) in IFA. Generation of anti-PrP T and B cell responses was analyzed in the spleen, lymph nodes, and serum of immunized C57BL/6 wild-type mice. Immunization with PrP peptides emulsified in CFA did not trigger an immune response to PrP. When CpG were used, vaccination with peptides P143–172 and P158–187 generated IFN-γ-secreting splenic T cells, and only P158–187 significantly stimulated IL-4-secreting T cells. Both peptides induced few Ab-producing B cells, and low and variable serum Ab titers. In contrast, immunization with peptide P98–127 did not induce significant levels of T cell responses but elicited specific peptide Abs. T cell epitope mapping, performed using 15-mer peptides covering PrP segment 142–182, revealed that an immunogenic motif lies between positions 156 and 172. These results demonstrate that T and B cell repertoires against PrP can be stimulated in C57BL/6 when adjuvant of the innate immunity such as CpG, but not CFA, is added to PrP peptides, and that the pattern of immune responses varies according to the epitope.
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