Isolation and characterisation of lead-resistant bacteria from a reverse screen model of simulation soil rice culture

CGMCC5515(Rif r ) were domesticated step by step.Pb-resistant bacteria (PbRB) of CGMCC5515 were selected in directional enrichment and screened with TTC from the pot rice rhizosphere soil with lead and other remediate material, the reverse screen model of simulation soil rice culture with reproducible TTC-detective method could be used as a tool in the R & D of bioremediate agent of lead-polluted soil.The PbRB with lead tolerance 1500 mg L -1 were identified as B. subtilis on the basis of morphological, biochemical and partial sequencing of their 16S rRNA and gyrB genes.Multiple stress tolerant CGMCC5515 immobilizated in alginate beads, formulated with biochar, attapulgite humic acid, reduced the pb of rice seeding to normal level.These results have reinforced the biotechnological potential of CGMCC5515 as a biological control agent.