视网膜色素上皮
转录组
细胞生物学
生物
地图集(解剖学)
上皮
视网膜
细胞
视网膜
解剖
计算生物学
神经科学
基因表达
遗传学
基因
植物
作者
Zongren Xu,Xian Liao,Na Li,Hongxiu Zhou,Hong Li,Qí Zhāng,Ke Hu,Peizeng Yang,Shengping Hou
标识
DOI:10.3389/fcell.2021.802457
摘要
Human retinal pigment epithelium cells are arranged in a monolayer that plays an important supporting role in the retina. Although the heterogeneity of specific retinal cells has been well studied, the diversity of hRPE cells has not been reported. Here, we performed a single-cell RNA sequencing on 9,302 hRPE cells from three donors and profiled a transcriptome atlas. Our results identified two subpopulations that exhibit substantial differences in gene expression patterns and functions. One of the clusters specifically expressed ID3 , a macular retinal pigment epithelium marker. The other cluster highly expressed CRYAB , a peripheral RPE marker. Our results also showed that the genes associated with oxidative stress and endoplasmic reticulum stress were more enriched in the macular RPE. The genes related to light perception, oxidative stress and lipid metabolism were more enriched in the peripheral RPE. Additionally, we provided a map of disease-related genes in the hRPE and highlighted the importance of the macular RPE and peripheral RPE clusters P4 and P6 as potential therapeutic targets for retinal diseases. Our study provides a transcriptional landscape for the human retinal pigment epithelium that is critical to understanding retinal biology and disease.
科研通智能强力驱动
Strongly Powered by AbleSci AI