NIR Mitochondrial Fluorescent Probe for Visualizing SO2/Polarity in Drug Induced Inflammatory Mice

SO₂ and polarity are important microenvironmental parameters in cells, which are closely related to physiological activities in organisms. The intracellular levels of SO₂ and polarity are abnormal in inflammatory models. To this end, a novel near-infrared fluorescent probe BTHP that can simultaneously detect SO₂ and polarity was studied. BTHP can sensitively detect polarity change with emission peak change from 677 to 818 nm. BTHP can also detect SO₂ with fluorescence change from red to green. After addition of SO₂, the fluorescence emission intensity ratio I₅₁₇/I₇₆₈ of the probe increased by about 33.6 times. BTHP can determine bisulfite in single crystal rock sugar with high recovery rate (99.2%-101.7%). Fluorescence imaging of cells showed that BTHP could better target mitochondria and monitor exogenous SO₂ in A549 cells. More importantly, BTHP has been successfully used for dual channel monitoring SO₂ and polarity in drug-induced inflammatory cells and mice. In particular, the probe showed increased green fluorescence with the generation of SO₂ and increased red fluorescence with the decrease of polarity in inflammatory cells and mice.