Simultaneous Determination of 30 Flavonoids in Jinqiancao Granules by LC–MS/MS

Abstract In order to determine the content of flavonoid components in Jinqiancao granules efficiently and accurately, a high-efficiency quantitative analysis method utilizing liquid chromatography–tandem mass spectrometry (LC–MS/MS) was employed. As per the guidelines from the International Council for Harmonization, a comprehensive method validation was conducted for this method. Experimental data demonstrated that the method exhibited excellent accuracy, specificity and reproducibility in the multiple reaction monitoring mode, facilitating the swift and accurate quantification of flavonoid content. Through chemometrics analysis, significant differences in the content of flavonoid compounds were observed among different batches of Jinqiancao granules samples. Isoschaftoside, Vicenin-2, Schaftoside and myricitrin can be used as the main quality markers for Jinqiancao granules. The research results provided a scientific basis for the quality control of Jinqiancao granules, which helps enhance the consistency and reliability of the product, thereby ensuring the superior quality of the preparation.