DNA甲基化
WRKY蛋白质结构域
生物
DNA
转录因子
遗传学
DNA结合位点
拟南芥
甲基化
基因
发起人
基因表达
突变体
作者
Magali Charvin,Thierry Halter,Romain Blanc-Mathieu,Pierre Barraud,Magali Aumont-Niçaise,François Parcy,Lionel Navarro
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2023-02-14
卷期号:192 (1): 77-84
被引量:4
标识
DOI:10.1093/plphys/kiad069
摘要
DNA methylation is an epigenetic mark that fine-tunes gene expression, notably by negatively or positively regulating transcription factor (TF)-DNA binding. In plants, DNA methylation has primarily been shown to inhibit TF-DNA binding. However, little is known about the underlying mechanisms. Here, we show that DNA methylation decreases the binding of several Arabidopsis (Arabidopsis thaliana) WRKY TFs to their genomic regions and their binding sites in vitro. We also provide evidence that DNA methylation at a single cytosine located in a functional core W-box motif repels DNA binding of AtWRKY40 in vitro. Using structural modelling, we further demonstrate that this cytosine interacts through van der Waals contacts with the conserved tyrosine of WRKY-DNA binding domains. Importantly, our model predicts steric hindrance when a 5-methyl group is present on this specific cytosine, thereby likely preventing tight binding of WRKY-DNA binding domains. Finally, because the WRKY motif and the residues involved in DNA contacts are conserved across Arabidopsis and rice (Oryza sativa) WRKY TFs, we propose that this methylation-dependent WRKY-DNA binding inhibitory mechanism could be widespread across plant species.
科研通智能强力驱动
Strongly Powered by AbleSci AI