化学
肽
计算生物学
质谱法
肽段
基质辅助激光解吸/电离
序列(生物学)
生物化学
色谱法
生物
有机化学
吸附
解吸
作者
Alfred L. Yergey,Jens R. Coorssen,Peter S. Backlund,Paul S. Blank,Glen A. Humphrey,Joshua Zimmerberg,J. M. Campbell,Marvin L. Vestal
标识
DOI:10.1016/s1044-0305(02)00393-8
摘要
The recently developed MALDI TOF-TOF instrument yields relatively complex but interpretable fragmentation spectra. When coupled with a straightforward sequence extension algorithm, it is possible to develop complete peptide sequences de novo from the spectra. This approach has been applied to a set of peptides derived from typtic digestion of electrophoretically separated sea urchin egg membrane proteins. When directed to proteins that have been described previously, the results were in essential agreement with those obtained by conventional data base searching approaches, with certain important exceptions. The present method detected errors in published sequences and was able to develop sequences from peptides differing in mass by one dalton (Da). These results show both the power of the present approach and the need for using de novo methods more frequently than may be otherwise appreciated.
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