哈卡特
明胶
角质形成细胞
组织谷氨酰胺转胺酶
人体皮肤
生物物理学
化学
粘附
基质(水族馆)
上皮
组织工程
自愈水凝胶
材料科学
细胞生物学
生物相容性材料
高分子化学
生物医学工程
生物化学
体外
酶
有机化学
生物
遗传学
生态学
医学
作者
Filippo Renò,Manuela Rizzi,M. Cannas
标识
DOI:10.1007/s10856-011-4519-9
摘要
Ionic hydrogels are biocompatible candidates for skin tissue engineering. Two hydrogels synthesized by crosslinking gelatin with polylysine (positively charged HG1) or polyglutamic acid (negatively charged HG2) were tested using spontaneously immortalized human keratinocytes (HaCaT). HaCaT cells displayed higher adhesion and proliferation onto HG2, forming a continuous and stratified epithelium after 7 days. Moreover HaCaT cells grown onto HG2 showed a decreased Epilysin and Filaggrin expression, while transglutaminase-1 expression was increased. Those data indicate that human keratinocyte can form a stratified epithelium onto HG2 that could therefore be an useful tool for skin tissue engineering.
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