热冲击系数
生物
热休克蛋白
拟南芥
转录因子
热休克蛋白70
热冲击
基因
细胞生物学
下调和上调
微阵列分析技术
小干扰RNA
发起人
高铁F1
蛋白质亚单位
拟南芥
抄写(语言学)
基因表达
遗传学
核糖核酸
突变体
哲学
语言学
作者
LI Shu-xia,Jinxin Liu,Zhongyuan Liu,Xiaorong Li,Feijie Wu,Yuqing He
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2014-04-01
卷期号:26 (4): 1764-1780
被引量:137
标识
DOI:10.1105/tpc.114.124883
摘要
Many heat stress transcription factors (Hsfs) and heat shock proteins (Hsps) have been identified to play important roles in the heat tolerance of plants. However, many of the key factors mediating the heat response pathways remain unknown. Here, we report that two genes, which are targets of TAS1 (trans-acting siRNA precursor 1)-derived small interfering RNAs that we named HEAT-INDUCED TAS1 TARGET1 (HTT1) and HTT2, are involved in thermotolerance. Microarray analysis revealed that the HTT1 and HTT2 genes were highly upregulated in Arabidopsis thaliana seedlings in response to heat shock. Overexpression of TAS1a, whose trans-acting small interfering RNAs target the HTT genes, elevated accumulation of TAS1-siRNAs and reduced expression levels of the HTT genes, causing weaker thermotolerance. By contrast, overexpression of HTT1 and HTT2 upregulated several Hsf genes, leading to stronger thermotolerance. In heat-tolerant plants overexpressing HsfA1a, the HTT genes were upregulated, especially at high temperatures. Meanwhile, HsfA1a directly activated HTT1 and HTT2 through binding to their promoters. HTT1 interacted with the heat shock proteins Hsp70-14 and Hsp40 and NUCLEAR FACTOR Y, SUBUNIT C2. Taken together, these results suggest that HTT1 mediates thermotolerance pathways because it is targeted by TAS1a, mainly activated by HsfA1a, and acts as cofactor of Hsp70-14 complexes.
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