乳过氧化物酶
化学
过氧化物酶
细胞色素c过氧化物酶
血红素
共振(粒子物理)
细胞色素c
血红素蛋白
拉曼光谱
共振拉曼光谱
辣根过氧化物酶
立体化学
过氧化氢
光化学
结晶学
酶
生物化学
物理
光学
粒子物理学
线粒体
作者
Catherine M. Reczek,Andres J. Sitter,James Terner
标识
DOI:10.1016/0022-2860(89)80004-3
摘要
Resonance Raman Fe(IV)=O stretching vibrations of intermediates of cytochrome c peroxidase and lactoperoxidase are identified on the basis of 18 O-induced frequency shifts. For cytochrome c peroxidase compounds ES a broad Fe(IV)=O peak centered at 753 cm −1 is identified by an 18 O-shift to 725 cm −1 . For lactoperoxidase compound II, a broad feature at 745 cm −1 , identified by spectral subtraction, undergoes an 18 O-induced shift to 712 cm −1 . Previous ferryl heme protein Fe(IV)=O frequencies have been found in the range from 770 to 800 cm −1 . The present Fe(IV)=O frequencies are thus the lowest Fe(IV)=O frequencies reported to date for ferryl (compound II type) heme enzyme intermediates and imply stronger hydrogen bond donation from distal amino acids to the oxo-ferryl group than for previously reported peroxidases. Concomitant with the lowered frequencies is a considerably higher Fe(IV)=O oxygen exchange efficiency with solvent water than previously observed for horseradish peroxidase compound II.
科研通智能强力驱动
Strongly Powered by AbleSci AI