The Study on Tissue Culture and Rapid Propagation of Ligularia fischeri

The tender stem of Ligularia fischeri was used as the explants to investigate its inducement and differentiation of callus,taking root of adventitious buds,transplantation of tube seedlings,and to establishment of rapid propagation technology for Ligularia fischeri.The results showed that the optimum medium for callus induction was MS+BA 0.3 mg/L+2,4-D 1.5 mg/L;the best medium for differentiation of callus and adventitious buds was MS+ BA 0.6 mg/L+NAA 0.1 mg/L;1/2MS+IAA 0.1 mg/L was suitable for rooting;the transplanted tube seedlings had some characteristics,such as tidy of plant,dark green leaf color,developed root system,meanwhile also kept all biological characteristics of the wild Ligularia fischeri.