Discussion and Analysis on Flammulina velutipes Polysaccharides Compositions

The polysaccharides from the fruiting body of Flammulina velutipes were extacted to evaluated the polysaccharides composition, molecular weight and monosaccharide constituents, which analyzed by DEAE Cellulose-52, high gel filtration chromatography( HPGFC) and PMP-HPLC. The results showed that the Flammulina velutipes polysaccharide( FVP) was composed of three faction, whose molecular weight(Mw) were 4 191 338, 372 779, 19 002. Among the three fraction of FVP compositions, the higher molecular weight of FVP composition was neutral polysaccharide, the other two frations were acid polysaccharide, meanwhile, the content of two acid polysaccharide frations were higher than the neutral polysaccharide fraction with44.31%, 37.76% and 17.93%, respectively. The FVP was composed of Glc, Man, Gal, Xyl, Fuc, and the Glc content was the highest, followed by Gal and Man, then Xyl and Fuc content was lowest. The molar ratio was relatively 13.05 ∶2.75 ∶3.16 ∶1.48 ∶1.00. In conclsion, the FVP might be composed of glucan mainly, which mixed by some fractions, such as galactose glycan,mannan, xylan and fucosan.