ANTI-GAAL antibody preparation, purification and identification

Objective To prepare anti-NGAL antibody with some purity and good immunoreactivity.Methods Animals were immunized with the fure NGAL protein and anti-NGAL serum was prepared. Then anti-NGAL antibody was purified by sedimentation with (NH4)2 SO4. At last purity of the anti-NGAL antibody was analyzed via SDS-PAGE and immunoreactivity of anti-NGAL antibody with NGAL protein was analyzed via Western blotting. Results After purified by sedimentation with (NH4 )2SO4 ,the purity of anti-NGAL antibody was more than 70% via SDS-PAGE and anti-NGAL antibody had good immnoreactivity with the pure NGAL protein by Western blotting. Conclusion Anti-NGAL antibody with definite purity and good immnoreactivity was gained at last.