Effects of simulated weightlessness on cell proliferation and cell cycle of cell line SGC-7901 and HFE-145

Objective To investigate the effects of simulated weightlessness on cell proliferation and cell cycle of human gastric carcinoma cell line SGC-7901 and human gastric mucosa cell line HFE-145. Methods A rotating clinostat was used to simulate weightlessness. Every cell line was divided into two groups, one was rotating group, the other was 1G control group. The whole experimental session was 72h. The expressions of PCNA(proliferating cell nuclear antigen) were examined by immunohistochemical stain. The changes of cell cycle were examined by a cytometer. Results Compared with the control group, the cell proliferation of SGC-7901 cell was inhibited in 48h and 72h in the rotating group, and the cell conversion from G1 to S phase was suppressed. Compared with the control group, the cell proliferation of HFE-145 cell was inhibited in 12h in the rotating group, and the cell conversion from G1 to S phase was suppressed. Conclusions the cell proliferation of SGC-7901 cell is inhibited in 48h and 72h during simulated weightlessness by a clinostat, but HFE-145 cells only have present those above-mentioned changes in 12h during simulated weightlessness by a clinostat.