Evaluation of the inactivation efficiency of the composite magnetic carrier photosensitizer by CPE determination with model virus as indicator

Objective To establish a method to determine TCID50,which can indicate the infection ability of virus,and then to use the method to evaluate the inactivation efficiency for model virus.Methods Pseudorabies virus was used as the model virus to infect the cultivated cells,and then cytopathic effect occurred.The experiment based on cytopathic effect and Reed Muench statistical method was used to determine the TCID50 of the virus,before and after the inactivation treatment,then the quantitative decrease was calculated.The inactivation conditions were optimized from three main factors: irradiation time,irradiation intensity and concentration of composite magnetic particles.Two kinds of material and different way of photochemical treatment was assayed to detect their influences to virus inactivation efficiency.Results After optimization,EMP-AA and EMP-AB played an excellent performance,and the decline value of PRV titer was more than 4 Lg TCID50.Conclusion The method for detecting the infection ability of virus is suitable to evaluate the efficiency of photochemical inactivation,and the result can actually reflect the capability of the material at the domain of virus inactivation.