Influence of the Concentrations of Hyaluronic Acid on the Adhesion and Proliferation of Human Fibroblasts

Hyaluronic acid,a component of the glycosaminoglycans in extracellular matrix,has a special physicochemical properties and influences several cellular functions such as adhesion,migration,and proliferation.The object of this study was to investigate the relationship between the concentrations of hyaluronic acid solutions and the adhesion and proliferation behaviors of human fibroblasts.Hyaluronic acid solutions with different concentrations were added to cell culture medium separately.Results demonstrated that it was only the concentration of hyaluronic acid solutions in a certain range(0.01%～0.1%),that could promote the cell adhesion,DNA synthesizing,GAG secreting and cell proliferation,while the concentrations of hyaluronic acid solutions were above 0.1% it would either reduce or even inhibit these behaviors.