ESTIMATED DIETARY INTAKE OF SOY ISOFLAVONES AND URINARY ISOFLAVONES

[Objective]To evaluate the relationship between dietary intake of soy isoflavones estimates and spot urine metabolites of soy isoflavones.[Methods]A total of 469 women of different communities in Nanjing completed the soy food frequency questionnaire(FFQ).The two kinds of soy isoflavones(genistein and daidzein)daily intake were determined by referring of the American food composition data.30 provided spot urine specimens,soybean isoflavones in urine metabolite concentrations,and these samples were detected by UPLC-MS/MS method.Genistein and daidzein in some selected soy food were analyzed by HPLC.[Results]Among all 469 women,mean genistein and daidzein intakes were 50.7±31.6mg/day and 33.4±35.9mg/day;The genistein and daidzein in urine specimens were as follows:genistein was 515.9±674.5 μmol/mol,and daidzein was 927.7±1107.2μmol/mol.Spot urine sample measures were correlated with soy FFQ estimates of genistein(Spearman r=0.59)and daidzein(Spearman r = 0.57)(P﹤0.05).[Conclusion]Estimates of genistein and daidzein has relationship with urine determination,which could provide solid data and method for study on the soy isoflavone and breast cancer.