Objective: To investigate the volatile constituents from 3 species flowers of Osmanthus fragrans Lour.Methods: The volatile constituents from the flowers of O.fragrans Lour.were analyzed by head-space solid microextraction,coupled with HS-SPME GC-MS for the first time.Results: 66 components were identified from the flowers of O.fragrans(Wanyingui),O.fragrans(Guifeihong) and O.fragrans(Yaotiaoshunv),the contents of which were determined respectively by peak area normalization method.The relative contents accounted to 79.96%、71.83% and 83.61% of peak area in the volatile group.Conclusion: The volatile constituents are investigated for the first time from different species,and it provides the basis for further development.