Two kinds of antibiotics resistance of lactic acid bacteria isolated from yogurt

Objective To investigate the ampicillin and tetracycline-resistant strains and resistant genes of lactic acid bacteria isolated from yogurt.Methods Lactic acid bacteria in yogurt were isolated and identified.Resistance to ampicillin or tetracycline of the strains were investigated with slip susceptibility test.Two pairs of primers were designed and ampicillin and tetracycline resistance gene were amplified.Results Seven lactic acid isolates were identified from 4 kinds of yogurt.A number of isolates were resistant to ampicillin or tetracycline.Five of the 7 lactic acid isolate contained ampicillin-resistant gene,and no isolate contained the tetracycline-resistant gene.Conclusion A number of lactic acid bacteria were resistant to antibiotics and could act as intermediate host of antibiotic resistant genes.