流式细胞术
滚动圆复制
核糖核酸
小RNA
分子生物学
细胞
染色
细胞生物学
生物
计算生物学
单细胞分析
基因
生物化学
聚合酶
遗传学
作者
Yansong Zhao,Chen Lin,Pengcheng Wu,Xiaoyuan Chen,Yuancun Zhao,Yupeng Li,Lu Chen,Mats Nilsson,Rongqin Ke
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2020-09-16
卷期号:5 (10): 3031-3036
被引量:6
标识
DOI:10.1021/acssensors.0c01569
摘要
Conventional flow cytometry has been widely used for high-throughput single-cell gene expression analysis using specific antibody staining. However, this is limited by the availability of high-quality antibodies. We developed a novel flow cytometry RNA detection technique termed RCA-Flow for single-cell RNA expression analysis. We showed that it is able to analyze not only mRNAs but also microRNAs and circular RNAs that are otherwise difficult to analyze by other flow cytometry techniques. The versatility for high-throughput analysis of different types of RNA molecules makes our method possess great potential for both biomedical and clinical applications.
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