Purification of PIA and rSesC as Putative Vaccine Candidates Against Staphylococcus aureus.

Staphylococcus aureus is predominant at sites of biomaterial-associated infection (BAI) and frequently infects hospitalized individuals.The polysaccharide intercellular adhesin (PIA) and S. epidermidis rSesC protein, major macromolecules in biofilm formation, were purified under native conditions and cloned and expressed in a prokaryotic host.LPurification of the macromolecules was confirmed by FTIR and Western blotting.The S. epidermidis SesC protein and PIA were uccessfully purified. Both are considered as vaccine candidates.