Purification of PIA and rSesC as Putative Vaccine Candidates Against Staphylococcus aureus.

BACKGROUND: is predominant at sites of biomaterial-associated infection (BAI) and frequently infects hospitalized individuals. METHODS: rSesC protein, major macromolecules in biofilm formation, were purified under native conditions and cloned and expressed in a prokaryotic host. RESULTS: LPurification of the macromolecules was confirmed by FTIR and Western blotting. CONCLUSION: SesC protein and PIA were uccessfully purified. Both are considered as vaccine candidates.